Porc Sauce Huitre (Recette Simple et Facile t. 1) (French Edition)
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What is the recommended concentration to use the antibody in western blot analysis? Where can I get the reactivity of your antibody with other species? How should I determine whether an antibody has cross-reactivity with other species?
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What immunogens are used in developing your antibodies? Can you list the immunogen sequence of your antibody? Can you please let me know how many biotins per antibody are bound? What is the IgG subtype for this polyclonal antibody? Can you please advise on the peak emission of the FITC? Why is the actual band greater than the theoretical molecular weight?
Why is the volume of this antibody I received less than that stated on the vial label? Can you recommend isotype control for the antibody? Can I get a free sample for a trial? What is the purity of this antibody? What is the common form of your antibodies? Which buffer is used to store antibodies? Which preservatives do you frequently use? Does the antibody contain any hazardous components? What kind of antibody labels can you offer?
Can you provide a bulk order of your antibodies for 1mg? What is the molecular weight of the antibody? Why are some antibodies no longer available? Why are there no bands in western blot WB? Why is the signal weak in western blot WB? Why are there extra bands in western blot WB? What is the reason for a smear in a western blot? Why does the band look diffuse and irregular in western blot WB? Why is transfer efficiency low in western blot WB?
Why is low resolution with high background across large regions in chromatin immunoprecipitation ChIP? You should perform a dot blot assay. The optimal dilution for ELISA should be determined by actual experiment depending on customer's assay platform and system. What is the reason for a smear in a western blot? You should empirically determine the conditions that preserve tissue morphology while restoring the immunoreactivity of the antigen.
Why is staining lack in immunohistochemistry IHC? Why is staining inappropriate in immunohistochemistry IHC? Why is background high in immunohistochemistry IHC? Why is tissue or cell morphology destroyed in immunohistochemistry IHC? Why is there too much antibody eluting in immunoprecipitation IP? Why is the background so high in immunoprecipitation IP? Why is low resolution with high background across large regions in chromatin immunoprecipitation ChIP? Why is high background in flow cytometry FC? Why are there two or more cell populations observed in flow cytometry FC? How long can I get the products after I place an order?
What can be the deliverables for your antibody service? How is the antibody packaged? Is it packaged in glass vial, plastic bottle or any other container? How should I store and aliquot the products? What is the shelf life of the products?
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We also offer custom antibody services , including custom recombinant antibody production , custom monoclonal antibody production , custom polyclonal antibody production and custom modified antibody production. Our official website has updated the articles from researchers who have published their results after using CUSABIO antibodies, Please click here to see more: As for quality control, we prove titers of antiserum and antibody by rigorous ELISA test, verify its specificity by western blot test or knockdown test, our antibody has broader scope of application and it's a powerful tool for scientific researchers to study their subjects.
We use in vivo ascites production for monoclonal antibodies, and serum production for polyclonal antibodies. Actually, we already have the technology for screening monoclonal antibodies by phage display technology. The rules you should pay attention to are as follows: CUSABIO guarantees its antibodies work for the applications and react with the species that listed on the website or product data sheet.
If you're working with an application that we did not test, we will offer you a trial size sample to evaluate the antibody before purchasing full size. Monoclonal antibodies are made using identical immune cells that are all clones of a specific parent cell. As such, they will have affinity for the same antigen and epitope.
Polyclonal antibodies are made using several different immune cells. They will have affinity for the same antigen but different epitopes. Monoclonal antibodies bind only a single epitope while polyclonal antibodies bind different epitopes on the same protein. Monoclonal antibodies are much more specific and with less background noise than polyclonal antibodies, and are as such generally preferable for biochemical assays.
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However, they are also usually more expensive to produce, less robust and are very sensitive to small changes in the antigen. In general, primary antibody is used to capture the target protein, secondary antibody can bind to primary antibody and is labeled with a enzyme or dye that can be amplified for detection. We suggest you consider the following elements:.
Depending on the host species. For example, if the primary antibody is from mouse, the secondary antibody should be anti-mouse. The optimal dilution for ELISA should be determined by actual experiment depending on customer's assay platform and system. You can blast the sequence of the immunogen with the sequence of your protein. Please download the protocol at https: Also, we can send you the protocol via e-mail if you can not find one for your experiment.
We use peptide, native protein and recombinant protein as immuogens. Please refer to the product information on our website or product data sheet for the immunogen sequence.
Recette Simple et Facile» est une collection de fiches «Recettes Cuisines du Monde». Édités par 22 Sud Éditions, en collaboration avec OIA Productions, cette. Porc Sauce Huitre (Recette Simple et Facile t. 1) (French Edition). 21 Feb | Kindle eBook. by Binado Mebisi and OIA Productions. £ Read this and.
If you can not find the immunogen sequence, please contact our technical support to request this information. Sorry, we have not checked the location of the epitope as it is not an essential check for many researches. You can identify the epitope by epitope mapping. The molar ratio of biotin to antibody is However, it's difficult to say exactly how many biotins are conjugated per antibody, as labeling is via the lysine residue and it's not clear how may primary amine groups are attached to biotin.
On average, biotin molecules can be conjugated per IgG molecule. Normally, polyclonal antibodies are raised in rabbit, and rabbit has only one IgG subtype. If there is a big difference between the test results and the theoretical molecular weight. The possible causes are as follows: The target protein has multiple modification sites such as glycosylation, or it forms stable complexes with other proteins in the body, or it is cut or degraded. Small volumes of antibody may occasionally become entrapped in the seal of the product vial during shipment and storage.
If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap. Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. Most isotype controls are monoclonal. Polyclonal antibodies are not suitable as these antibodies contain more than one IgG subclass.
Click here to learn more about the antibody promotion. Please let us have the lot number. To the best of our knowledge, none of the components presents in this product is classified as hazardous. In general, most common type of our antibodies is IgG with a molecular weight of approximately kDa. We remove these antibodies from our stock because we have new products replacement.
Antibody Ab , also known as immunoglobulin Ig , is a Y-shaped protein that is used by the immune system to neutralize pathogens such as pathogenic bacteria and viruses via the Fab's variable region. Some antibodies may have poor affinity to target proteins. You should reduce antibody dilution fold lower than recommended starting dilution. Meanwhile, antibody may have lost activity. You should perform a dot blot assay.
You should increase the amount of total protein loaded on gel. Confirm the presence of protein by another method. Use a positive control recombinant protein, cell line or treat cells to express analyte of interest. Perform a dot blot assay. You should extend transfer time, because some proteins with high molecular weight may require some more time on transfer.
You should reduce transfer voltage or time for those proteins with small molecular weight lower than 10 kDa. Isoelectric point is bigger than 9. Incorrect secondary antibody used. You should confirm host species and antibody type of primary antibody to choose the right secondary antibody. You should make sure buffers do not contain Sodium azide for it can quench HRP signal.
Insufficient incubation time for primary antibody. You should minimize the number of washes. Reduce NaCl concentration in antibody solution or use blotting buffer for wash steps recommended range 0. You should increase antibody concentration to fold higher than recommended starting concentration. You should mix enzyme and substrate in a tube. If color does not develop or, it is weak, make fresh or purchase new reagents.
Non-fat dry milk may mask some antigen. Non-specific binding of primary antibody. You should increase primary antibody dilution factor. Reduce the amount of total protein loaded on gel. Use mono-specific or antigen affinity purified antibodies.
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Non-specific binding of secondary antibody. You should run a control with the secondary antibody alone omit primary antibody. If bands develop, then choose an alternative secondary antibody, use mono-specific or antigen affinity purified antibodies. Heat in boiling water for minutes before loading onto gel. Perform a short-time centrifugation.
You should check whether the buffers had been contaminated or not, or make fresh reagents alternatively. If there are some bands appearing on the membrane, you should dilute the antibody or change another secondary antibody alternatively. Incubation time could be extended. Adjust milk concentration up or down based on needs. You should increase the concentration of Tween 20 in wash buffer 0. You should reduce exposure time.
If target signal is too strong, then please wait minutes more and re-expose to film. Some samples have higher salt concentrations.
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You should desalt or adjust the salt concentration of the sample. Inconsistent sample volume per well. You should adjust the sample volume so that it is basically the same. Air bubble trapped in membrane. Le tout s'accompagne des muffins , des fruits , mais le plus souvent du toast: Des boissons comme le jus d'orange, de pomme, d'ananas ou du jus de canneberge accompagnent le repas.
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